Characterization of the protein expression and localization of hnRNP family members during murine spermatogenesis / 亚洲男科学杂志(英文版)

Mammalian testis exhibits remarkably high transcriptome complexity, and spermatogenesis undergoes two periods of transcriptional cessation. These make the RNA-binding proteins (RBPs) the utmost importance during male germ cell development. Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a large family of RBPs implicated in many steps of RNA processing; however, their roles in spermatogenesis are largely unknown. Here, we investigated the expression pattern of 12 hnRNP family members in mouse testes and found that most detected members are highly expressed in the testis. Furthermore, we found that most of the detected hnRNP proteins (hnRNPD, hnRNPK, hnRNPQ, hnRNPU, and hnRNPUL1) display the highest signals in the nuclei of pachytene spermatocytes, round spermatids, and Sertoli cells, whereas hnRNPE1 exclusively concentrates in the manchette of elongating spermatids. The expression of these hnRNP proteins showed both similarities and specificity, suggesting their diverse roles in spermatogenesis.

Effects of chrysophanol on expression of SREBPs and lipid metabolism in Huh-7 cells / 药学学报

Rhubarb is a traditional Chinese medicines which possess laxative, lipid-lowering, and weight-loss activities, but the active compounds of lipid-lowering and underlying molecular mechanisms are not yet clear. This study aims to explore the effects of chrysophanol on the mRNA expressions of sterol regulatory element-binding proteins (SREBPs) and lipid metabolism in human liver carcinoma Huh-7 cells, which is one of the active compounds obtained from Rhubarb. A reporter gene assay was used to test the transcription of SREBP. The intracellular triglyceride and total cholesterol contents were measured by using commercially available test kits. The SREBPs target genes expressions were measured by Quantitative Real-Time PCR. Cell viability was evaluated by Cell Counting Kit-8. As the results shown, chrysophanol (40 μmol · L(-1), 16 h) could notably inhibited human SRE promoter activity in a dose-dependent manner and decrease intracellular cholesterol and triglyceride levels. Furthermore, the mRNA expressions of SREBPs target genes were significantly downregulated by chrysophanol treatment. However there are no significant differences on cell viability when compared with the control group. These results suggested that chrysophanol might improve lipid metabolism through suppressing the mRNA expressions of SREBPs target genes to attenuate intracellular lipid accumulation.

Factors associated with epileptic seizure of cavernous malformations in the central nervous system in West China

Objective: To explore the factors associated with preoperative and postoperative epileptic seizure in patients with cavernous malformations [CMs]

Methods: A total of 52 consecutive patients from January 2009 to June 2011 who underwent surgical treatment in West China Hospital of Sichuan University due to CMs and confirmed by histopathology were retrospectively reviewed.Patients were divided into two groups [epilepsy-group and non-epilepsy group] according to clinical presentation. Other clinical data, treatment procedure, and follow-up information were collected. Engel classification was used to evaluate seizure outcome

Results: Low birth weight, temporal lobe involvement and cortical lesion showed significant difference between two groups [p=0.017, 0.003 and 0.025 respectively]. Cortical lesion highly increased risk for preoperative epileptic seizure [OR=10.48; 95% CI 1.61-68.23]. After a mean follow-up of 2.1 years, 77.8% of epileptic patients achieved Engel class I. Temporal lobe involvement, lesion size < 2.5cm and surgery within one year of symptom onset were found associated with better seizure outcome [p=0.016, 0.012 and 0.050]. Temporal lobe involvement significantly decreased the risk for postoperative epileptic seizure [OR=0.038; 95% CI 0.002-0.833]. Application of ECoG made no significant difference to seizure outcome [p=0.430]. Most patients need continuing medication therapy after surgery

Conclusion: Surgical treatment of patient with CMs is satisfactory in most cases and temporal lobe involvement usually predict favourable postoperative seizure outcome whether under the monitoring of ECoG or not. Thus, epileptic patients with CMs should be considered for surgical treatment especially when cortical brain layer or temporal lobe was involved

The expressions of CDC25A and CDC25B in esophageal squamous cell carcinoma and their relationship with cell apoptosis and proliferation / 肿瘤

Objective: To investigate the biological significance of the expressions of CDC25A and CDC25B and their relation to cell apoptosis and proliferation of esophageal squamous cell carcinoma. Methods: The expressions of CDC25A and CDC25B were examined by SP immunohistochemical stain (IHC) in 52 cases of esophageal carcinoma specimens, 25 cases of dysplasia and 24 cases of normal tissues. The apoptotic ratio, proliferation index (PI), the expression of CDC25A and CDC25B protein were determined by FCM in 30 cases with esophageal carcinoma and 5 cases of normal tissues. Results: The positive expression rates of CDC25A (50%) and CDC25B (48.1%) in esophageal carcinoma were significantly higher than those in dysplasia(16%) and normal tissues (0%) (P < 0.01); the expressions of CDC25A and CDC25B were correlated with differentiation degree and invasion depth of tumor cells, and CDC25A was related with lymph node metastasis of esophageal squamous cell carcinoma. The PI was significantly higher in nuclear membrane protein CDC25A-positive group than that in nuclear membrane protein CDC25A-negative group (P < 0.05). Conclusion: CDC25A is involved in the carcinogenesis and progression of esophageal squamous cell carcinoma and has the potential to become a new biological marker to predict the carcinogenesis and prognosis of esophageal carcinoma; CDC25B might play a role in the early phase of esophageal squamous cell carcinoma. The CDC25A protein in the nuclear membrane might contribute to cell proliferation.

In vitro stimulation of retinoic acid syndrome by rotary cell culture system and its relationship with expression of CXCR4 and SDF-1 alpha / 中华血液学杂志

<p><b>OBJECTIVE</b>To explore the molecular mechanism and prevention of retinoic acid syndrome (RAS).</p><p><b>METHODS</b>SDF-1 alpha mRNA from healthy adult lung tissue was measured by RT-PCR, CXCR4 protein expression on the cell membrane of APL cells induced by ATRA (APL-ATRA) was tested by FCM, and the rotary cell culture system (RCCS) was used to build a modal for in vitro stimulation of APL-ATRA infiltrating human lung tissue. The ability of APL-ATRA in adhesion, migration and infiltration was observed by interference from DEX, Ara-C and DNR.</p><p><b>RESULTS</b>The APL-ATRA cells could evidently infiltrate into normal lung tissue. Mean fluorescence intensity (MFI) of CXCR4 on the cell membrane of APL-ATRA cells was 30.6 +/- 1.8, which was much higher than that on unspecialized APL cells (9.8 +/- 4.2). SDF-1 alpha mRNA expression was detected positive in all 6 lung tissue. Contrary to the control groups, DEX could dramatically restrain the ability of APL-ATRA cells in adhesion and migration [(27.2 +/- 2.6)% vs. (46.0 +/- 3.0)%, (28.1 +/- 4.0)% vs. (48.2 +/- 3.0)%], while Ara-C and DNR could distinctly depress the ability in adhesion, migration and infiltration [(28.1 +/- 3.0)%, (30.2 +/- 3.2)% vs. (46.0 +/- 3.0)%; (29.0 +/- 4.0)%, (23.0 +/- 5.2)% vs. (48.2 +/- 3.0)%; (16.8 +/- 7.6)%, (17.1 +/- 6.0)% vs. (43.6 +/- 5.0)%].</p><p><b>CONCLUSION</b>In vitro APL-ATRA cells can infiltrate into the human lung tissue. High expression of CXCR4 on APL-ATRA and SDF-1 alpha in the lung tissue may be one of the molecular mechanisms of the lung infiltration and RAS. DEX, Ara-C and DNR can dramatically restrain the ability of APL-ATRA cells in adhesion, migration and infiltration.</p>

Investigation of Sleep Status and Effect of Night Shift Work on Sleep in Nurses / 中国康复理论与实践

@#ObjectiveTo investigate the sleep status and effect of night shift work on sleep in nurses.MethodsSleep characteristics and qualities of 348 nurses, 50 communication persons and 100 night-guards were analyzed with Pittsburgh Sleep Quality Index (PSQI), and insomnia was diagnosed based on DSM-IV criteria.Results71.86% of nurses had bad sleep quality (PSQI>7); the rate of insomnia was 38.22%. The same data were 74% and 48% respectively in communication persons, and 26.76% and 7% in night-guards. The sleep quality of nurses was related to age and years of night shift work. The sleep quality of nurses was similar to communication persons (P>0.05), but significantly different from that of night-guards (P<0.01).ConclusionShift work manner influences sleep status of nurses and makes them having disturbances on falling sleep time, the time of sleeping, sleep efficiency and daytime function. But it doesn't need medication.

Expression of cyclin dependent kinases-5 in the temporal lobes of the drug resistance epilepsy patients / 中华神经科杂志

Objective To investigate the expression of cyclin dependent kinases 5(CDK5)in the temporal lobes of the epilepsy patients and to explore the possible roles of CDK5 in the pathogenesis of refractory epilepsy.Methods The brain tissues of intractable epilepsy(IE)were studied by fluorescence quantative polymerase chain reaction(FQ-PCR)for CDK5 mRNA,while immunohistochemistry and Western blot were used to study the protein expression.Nonepileptogenic control brain tissues were used for comparison.Results FQ-PCR analysis showed that the expression of CDK5 mRNA in epilepsy patients was significant higher than those in the control group.And immunohistochemistry showed that the protein mainly existed in the neuron and glial.At the 35000 relative molecular mass,Western blot could been seen that there is a limpid strap.The optical density of CDK5 in IE(temporal lobe 1.4293?0.1839,hippocampus 2.0733?0.4738)was significantly higher than that in the control(temporal lobe 0.9680?0.4147, hippocampus 1.4030?0.6160,P